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Report Delivery
1 Day
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Free Sample Collection
Bookings above 500
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Pre - Instruction
No Preparation Required.
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Covid Safety
Assured
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| Test Details |
| Test Code |
BOBT00520 |
| Test Category |
Individual Test |
| Sample Type |
Blood |
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Details of Hepatitis C Virus Antibody (HCV) - ELISA
What is Hepatitis C Virus Antibody (HCV) - ELISA?
All commercial Hepatitis C virus antibody (anti-HCV) assays use a combination of recombinant antigens to detect antibody response. Antibody responses to individual antigenic regions (core, NS3/4, and NS5) used in assays have not been investigated.
Methods
In this study, we quantified HCV viral load, tested anti-HCV with four commercial assays (Ortho-ELISA, Murex-ELISA, Architect-CMIA, and Elecsys-ECLIA) in 682 plasma specimens. In the antigenic region ELISA platform, microwells were coated with three antigens: core (c22-3), NS3/4 (c200) and NS5 individually. The signal-to-cutoff (S/Co) values of different assays and antibody responses to individual antigens were compared. The specimens were divided into HCV RNA positive group, anti-HCV consistent group, and anti-HCV discrepant group.
Results
Anti-core and anti-NS3/4 were simultaneously detected in 99.2% of HCV RNA-positive specimens and showed great consistency with total anti-HCV signals. Responses to the core region were more robust than those to the NS3/4 region in the anti-HCV consistent group (p?Conclusion
Antibody responses to the core and NS3/4 antigens were stronger, whereas responses to the NS5 antigen were the weakest, indicating that individual antigenic regions played different roles in total anti-HCV signals. This study provides an impetus for optimizing commercial anti-HCV assays.
