What is Immunofixation Electrophoresis - Qualitative ?
Also Known As:
Serum Protein Electrophoresis, Protein ELP, SPE, SPEP, Gel Electrophoresis, Capillary Electrophoresis, Immunosubtraction Electrophoresis, Urine Protein Electrophoresis, UPE, UPEP, IFE, CSF Protein Electrophoresis, Electrophoresis
Protein Electrophoresis, Immunofixation Electrophoresis
Proteins are important building blocks of all cells and tissues. They form the structural part of most organs and make up enzymes and some hormones that regulate bodily functions. Protein electrophoresis is a lab method that separates proteins based on their size and electrical charge. Knowing which proteins are present, absent, elevated, or decreased in body fluids such as blood can help diagnose and/or monitor various diseases and conditions.
Body fluids, such as blood, urine, and cerebrospinal fluid (CSF) contain many different proteins that have various roles, such as transporting nutrients, removing toxins, and controlling body functions.
The two major types of proteins are:
- Albumin is produced in the liver and accounts for about 60% of the protein in the blood.
- Globulins is a collective term used to refer to proteins other than albumin. With the exception of antibodies (immunoglobulins) and some complement proteins, most of the globulins are also produced in the liver.
The various proteins in body fluids are subjected to a controlled electric current, fractionating them into a typical pattern of bands or peaks that then can be measured. The proteins are divided into six groups, called prealbumin (rarely detected on serum or urine protein electrophoresis), albumin, alpha 1, alpha 2, beta, and gamma. The beta fraction may be further divided into beta 1 and beta 2 subgroups.
Each of these protein groups (electrophoresis fractions) is distinct and at specific concentrations. The patterns typically seen in certain conditions and diseases can help with diagnosis. The major serum proteins and their functions are listed according to their electrophoretic region or fraction (the visible band that they are part of) in the table titled Protein Groups.
Various conditions and diseases can affect protein production and/or protein loss, thus changing the pattern of bands seen on a protein electrophoresis. The most important example is the appearance of a distinct, monoclonal band in the gamma region. For example, uncontrolled growth and division of a malignant plasma cell as seen in multiple myeloma leads to the production of large amounts of a single type of immunoglobulin (monoclonal immunoglobulin).
When an abnormal protein (band or peak) is detected, additional tests are done to identify the type of protein (immunotyping). Immunofixation electrophoresis or immunosubtraction electrophoresis can be used to identify abnormal bands seen on protein electrophoresis, typically in the gamma region, in order to determine whether a type of antibody (immunoglobulin) is abnormally produced (e.g., IgG, IgA, IgM).
In most cases of multiple myeloma, a single type of intact (whole) immunoglobulin is produced in excess. In a minority of cases, only one section of an immunoglobulin called a “free light chain” is produced in large amounts. These excess free light chains are released into the bloodstream and since they are relatively small molecules, they are filtered by the kidneys and released into the urine. Another term for these excess free light chains in the urine is Bence Jones proteins.