What is Leptospira Antibody IgG - ELISA?

Leptospira infections in humans are transmitted from infected animals, or by exposure to areas inhabited by contaminated animals. Another source of infection is derived from swimming or bathing in contaminated water, as the infection enters through mucosal eye surfaces or skin contusions. Infected animals may include rats and mice, but also larger mammals (such as dogs, sheep, cows, deer, rabbits, etc.) are primary sources of infection. The leptospira bacteria are shed through the urine of infected animals. The incubation period of the infection in humans is normally 10 to 12 days, but can also range from 3 to 30 days. Antibody detection occurs around the 6th to 10th day of disease, and within 3 to 4 weeks, they reach their peak level. Even though antibody levels slowly abate, they may persist and show up in diagnosis years afterward. The extent of symptoms can range anywhere from a mild inflammation of the mucous membrane to jaundice, where severe kidney and liver infections are detected. The Leptospira ELISA kit is the ideal test for acute leptospirosis infections.

Leptospira IgG IgM ELISA Test Principle:

The principle of the Leptospira ELISA test is a three-incubation process with the first incubation involving the coating of the wells with purified Leptospira Patoc1 antigen. Next, the wells must be washed to remove the test sample, and then Enzyme Conjugate is added. In the presence of this enzyme complex and peroxide, a substrate is added, and as a result of this combination, the substrate color will turn blue. The reaction is ended with the stop solution, and what appears is a yellow color instead of blue.