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MTB PCR by Gene Xpert
Parameters : 1
Also known as : MTB PCR by Gene Xpert
EXCLUSIVE PRICE
4000
Report Delivery
4 Days
Free Sample Collection
Bookings above 500
Pre - Instruction
No Preparation Required.
Covid Safety
Assured
Test Details
Test Code BOBT00689
Test Category Individual Test
Sample Type Sputum
Details of MTB PCR by Gene Xpert
What is the Xpert MTB/RIF Assay?
The Xpert MTB/RIF assay is a new test that is revolutionizing tuberculosis (TB) control by contributing to the rapid diagnosis of TB disease and drug resistance. The test simultaneously detects Mycobacterium tuberculosis complex (MTBC) and resistance to rifampin (RIF) in less than 2 hours. In comparison, standard cultures can take 2 to 6 weeks for MTBC to grow and conventional drug resistance tests can add 3 more weeks. The information provided by the Xpert MTB/RIF assay aids in selecting treatment regimens and reaching infection control decisions quickly.
How Does the Xpert MTB/RIF Assay Work?
The Xpert MTB/RIF assay is a nucleic acid amplification (NAA) test that uses a disposable cartridge with the GeneXpert Instrument System. A sputum sample is collected from the patient with suspected TB. The sputum is mixed with the reagent that is provided with the assay, and a cartridge containing this mixture is placed in the GeneXpert machine. All processing from this point on is fully automated.
What are the Advantages of the Xpert MTB/RIF Assay?
Major advantages of the Xpert MTB/RIF assay are that
  • Results are available quickly, and
  • Minimal technical training is required to run the test.
  • Additionally, the Xpert MTB/RIF assay can quickly identify possible multidrug-resistant TB (MDR TB). MDR TB is TB that is resistant to both isoniazid (INH) and RIF, two of the most effective TB drugs. RIF resistance is a predictor of MDR TB because resistance to RIF, in most instances, co-exists with resistance to INH. Rapid diagnosis of RIF resistance potentially allows TB patients to start effective treatment much sooner than waiting for results from other types of drug susceptibility testing.
For patients who are found to NOT have TB disease, rapid results from the Xpert MTB/RIF assay may contribute to cost savings by avoiding unnecessary treatment and respiratory isolation in hospitals or other institutions.
How Should Xpert MTB/RIF Assay Results be Interpreted?
As with other NAA tests, the Xpert MTB/RIF assay should be interpreted along with clinical, radiographic, and other laboratory findings. The Xpert MTB/RIF assay does not replace the need for a smear with microscopy for acid-fast bacilli, culture for mycobacteria, and growth-based drug susceptibility testing, in addition to genotyping for early discovery of outbreaks. Providers and laboratories need to ensure that patient specimen are available for recommended mycobacterial testing.

Results from the Xpert MTB/RIF assay indicate whether or not MTBC was detected in the sample. In some instances, the result is “invalid,” whereby the test should be repeated. If MTBC was detected, the results will also state whether resistance to RIF was
  • Detected,
  • Not detected, or
  • Indeterminate.
Regardless of the Xpert MTB/RIF result, patient specimens should also have a mycobacterial culture to ensure isolates are available for drug susceptibility testing and genotyping.
  • RIF Resistance Detected
Results that are positive for MTBC and for RIF resistance mean that the bacteria have a high probability of resistance to RIF. This should be confirmed by additional rapid testing. If RIF resistance is confirmed, rapid molecular testing for drug resistance to both first-line and second-line drugs should be performed so that an effective treatment regimen can be selected. CDC offers rapid molecular detection of drug resistance (MDDR) services through public health laboratories, free of charge.
Routine Tests
MTB PCR by Gene Xpert
Parameters : 1
Also known as : MTB PCR by Gene Xpert
EXCLUSIVE PRICE
4000
Report Delivery
4 Days
Free Sample Collection
Bookings above 500
Pre - Instruction
No Preparation Required.
Covid Safety
Assured
Test Details
Test Code BOBT00689
Test Category Individual Test
Sample Type Sputum
Details of MTB PCR by Gene Xpert
What is the Xpert MTB/RIF Assay?
The Xpert MTB/RIF assay is a new test that is revolutionizing tuberculosis (TB) control by contributing to the rapid diagnosis of TB disease and drug resistance. The test simultaneously detects Mycobacterium tuberculosis complex (MTBC) and resistance to rifampin (RIF) in less than 2 hours. In comparison, standard cultures can take 2 to 6 weeks for MTBC to grow and conventional drug resistance tests can add 3 more weeks. The information provided by the Xpert MTB/RIF assay aids in selecting treatment regimens and reaching infection control decisions quickly.
How Does the Xpert MTB/RIF Assay Work?
The Xpert MTB/RIF assay is a nucleic acid amplification (NAA) test that uses a disposable cartridge with the GeneXpert Instrument System. A sputum sample is collected from the patient with suspected TB. The sputum is mixed with the reagent that is provided with the assay, and a cartridge containing this mixture is placed in the GeneXpert machine. All processing from this point on is fully automated.
What are the Advantages of the Xpert MTB/RIF Assay?
Major advantages of the Xpert MTB/RIF assay are that
  • Results are available quickly, and
  • Minimal technical training is required to run the test.
  • Additionally, the Xpert MTB/RIF assay can quickly identify possible multidrug-resistant TB (MDR TB). MDR TB is TB that is resistant to both isoniazid (INH) and RIF, two of the most effective TB drugs. RIF resistance is a predictor of MDR TB because resistance to RIF, in most instances, co-exists with resistance to INH. Rapid diagnosis of RIF resistance potentially allows TB patients to start effective treatment much sooner than waiting for results from other types of drug susceptibility testing.
For patients who are found to NOT have TB disease, rapid results from the Xpert MTB/RIF assay may contribute to cost savings by avoiding unnecessary treatment and respiratory isolation in hospitals or other institutions.
How Should Xpert MTB/RIF Assay Results be Interpreted?
As with other NAA tests, the Xpert MTB/RIF assay should be interpreted along with clinical, radiographic, and other laboratory findings. The Xpert MTB/RIF assay does not replace the need for a smear with microscopy for acid-fast bacilli, culture for mycobacteria, and growth-based drug susceptibility testing, in addition to genotyping for early discovery of outbreaks. Providers and laboratories need to ensure that patient specimen are available for recommended mycobacterial testing.

Results from the Xpert MTB/RIF assay indicate whether or not MTBC was detected in the sample. In some instances, the result is “invalid,” whereby the test should be repeated. If MTBC was detected, the results will also state whether resistance to RIF was
  • Detected,
  • Not detected, or
  • Indeterminate.
Regardless of the Xpert MTB/RIF result, patient specimens should also have a mycobacterial culture to ensure isolates are available for drug susceptibility testing and genotyping.
  • RIF Resistance Detected
Results that are positive for MTBC and for RIF resistance mean that the bacteria have a high probability of resistance to RIF. This should be confirmed by additional rapid testing. If RIF resistance is confirmed, rapid molecular testing for drug resistance to both first-line and second-line drugs should be performed so that an effective treatment regimen can be selected. CDC offers rapid molecular detection of drug resistance (MDDR) services through public health laboratories, free of charge.
 

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